phtc halotag (ht) cmv-neo vector  (Promega)

Journal: Oncology Letters

Article Title: CD146 promotes migration and proliferation in pulmonary large cell neuroendocrine carcinoma cell lines

doi: 10.3892/ol.2018.9830

Figure Lengend Snippet: CD146 expression increases the migratory ability of large cell neuroendocrine carcinoma cells. (A) Migration of NCI-460 cells following transfection with control or CD146-specific siRNAs. (B) Migration of NCI-460 cells following plasmid transfection. (C) Migration of NCI-810 cells following plasmid transfection. Representative photomicrographs are presented for each group (scale bar, 100 µm; magnification, ×100). The bar charts show quantification of the cell migration. The bars represent the mean cell counts ± standard deviation, and are normalized to the control group. The experiment was performed three times. *P<0.05, vs. si-control or HT alone group). CD146, cluster of differentiation 146; si/siRNA, small interfering RNA; HT, HaloTag vector.

Article Snippet: NCI-H460 and NCI-H810 cells were transiently transfected with this plasmid (0.015 µg/µl) or a HaloTag (HT) control vector (0.015 µg/µl; cat. no. G6591; Promega Corporation) using Fugene ® HD transfection reagent (Promega Corporation), according to the manufacturer's protocol ( ).

Techniques: Expressing, Migration, Transfection, Plasmid Preparation, Standard Deviation, Small Interfering RNA

Journal: Oncology Letters

Article Title: CD146 promotes migration and proliferation in pulmonary large cell neuroendocrine carcinoma cell lines

doi: 10.3892/ol.2018.9830

Figure Lengend Snippet: CD146 expression is correlated with EMT marker levels. (A) Western blot showing the levels of EMT marker proteins in NCI-460 cells following siRNA transfection. (B) Western blot showing the levels of EMT maker proteins in NCI-460 and NCI-810 cells following transfection with CD146 expression plasmid. Each experiment was performed three times. *P<0.05, vs. si-control or HT alone group. CD146, cluster of differentiation 146; EMT, epithelial-mesenchymal transition; si/siRNA, small interfering RNA; HT, HaloTag vector.

Article Snippet: NCI-H460 and NCI-H810 cells were transiently transfected with this plasmid (0.015 µg/µl) or a HaloTag (HT) control vector (0.015 µg/µl; cat. no. G6591; Promega Corporation) using Fugene ® HD transfection reagent (Promega Corporation), according to the manufacturer's protocol ( ).

Techniques: Expressing, Marker, Western Blot, Transfection, Plasmid Preparation, Small Interfering RNA

Journal: Oncology Letters

Article Title: CD146 promotes migration and proliferation in pulmonary large cell neuroendocrine carcinoma cell lines

doi: 10.3892/ol.2018.9830

Figure Lengend Snippet: CD146 expression is associated with AKT activity. (A) Western blot and quantified results showing AKT phosphorylation level in NCI-460 cells following siRNA transfection. (B) Western blot and quantified results showing AKT phosphorylation level in NCI-460 and NCI-810 cells following transfection with CD146 expression plasmid. Each experiment was performed three times. *P<0.05, vs. si-control or HT alone group. CD146, cluster of differentiation 146; si/siRNA, small interfering RNA; HT, HaloTag vector; p-, phosphorylated.

Article Snippet: NCI-H460 and NCI-H810 cells were transiently transfected with this plasmid (0.015 µg/µl) or a HaloTag (HT) control vector (0.015 µg/µl; cat. no. G6591; Promega Corporation) using Fugene ® HD transfection reagent (Promega Corporation), according to the manufacturer's protocol ( ).

Techniques: Expressing, Activity Assay, Western Blot, Transfection, Plasmid Preparation, Small Interfering RNA